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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
07/11/2011 |
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Data da última atualização: |
07/11/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SCHEUERMANN, K. K.; BROMMONSCHENKEL, S. H. |
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Afiliação: |
Epagri |
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Título: |
A stronger and faster defense response is associated with age-related early blight resistance in tomato. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 13., 2007, Sorrento, Itália. [Proceedings...]. Itália: [s.n.], 2007. p. 321-322. |
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Idioma: |
Inglês |
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Conteúdo: |
The early blight, caused by the necrotrophic fungi Alternaria solani, is an important disease of the tomato, causing significant yield losses. The development of resistant cultivars has been difficult because the resistance is polygenic and germplasm with adequate levels of resistance is not available. In addition to, resistance is negatively correlated with physiological maturity and fruit load of the plant; older senescing leaves are more susceptible than immature leaves, and a high fruit set enhances the disease. In order to identify genes involved with quantitative resistance against A. solani, cDNA libraries were built using the suppressive subtraction hybridization technique (SSH). Sequence analysis of 1536 ESTs allowed the identification of cDNA clones with similarities with genes involved in plant defense, gene expression regulation and signals transducition pathways. Among the putative genes related with defense machanisms, there is a predominance of genes reported to be regulated by ethylene and jasmonic acid and a lower frequency of ESTs with similarity to genes known to be regulated by salicylic acid. Real time PCR analysis of selected genes revealed that 40% of them are induced in response to Alternaria solani. Transcripts of the majority of these genes were detected between 6 and 12 hai, reaching a maximum value between 24 and 36 hai. Several genes showed a high level of expression and a faster expression response in younger leaves where the disease is less severe. The higher level of defense gene expression associated whith a faster defense response can be one of the mechanisms responsible for the reduced level of early blight observed in yourger tomato leaves. MenosThe early blight, caused by the necrotrophic fungi Alternaria solani, is an important disease of the tomato, causing significant yield losses. The development of resistant cultivars has been difficult because the resistance is polygenic and germplasm with adequate levels of resistance is not available. In addition to, resistance is negatively correlated with physiological maturity and fruit load of the plant; older senescing leaves are more susceptible than immature leaves, and a high fruit set enhances the disease. In order to identify genes involved with quantitative resistance against A. solani, cDNA libraries were built using the suppressive subtraction hybridization technique (SSH). Sequence analysis of 1536 ESTs allowed the identification of cDNA clones with similarities with genes involved in plant defense, gene expression regulation and signals transducition pathways. Among the putative genes related with defense machanisms, there is a predominance of genes reported to be regulated by ethylene and jasmonic acid and a lower frequency of ESTs with similarity to genes known to be regulated by salicylic acid. Real time PCR analysis of selected genes revealed that 40% of them are induced in response to Alternaria solani. Transcripts of the majority of these genes were detected between 6 and 12 hai, reaching a maximum value between 24 and 36 hai. Several genes showed a high level of expression and a faster expression response in younger leaves where the disease is less sev... Mostrar Tudo |
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Palavras-Chave: |
Alternaria solani; Early blight. |
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Categoria do assunto: |
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Marc: |
LEADER 02238naa a2200145 a 4500
001 1081821
005 2011-11-07
008 2007 bl uuuu u00u1 u #d
100 1 $aEpagri
245 $aA stronger and faster defense response is associated with age-related early blight resistance in tomato.
260 $c2007
520 $aThe early blight, caused by the necrotrophic fungi Alternaria solani, is an important disease of the tomato, causing significant yield losses. The development of resistant cultivars has been difficult because the resistance is polygenic and germplasm with adequate levels of resistance is not available. In addition to, resistance is negatively correlated with physiological maturity and fruit load of the plant; older senescing leaves are more susceptible than immature leaves, and a high fruit set enhances the disease. In order to identify genes involved with quantitative resistance against A. solani, cDNA libraries were built using the suppressive subtraction hybridization technique (SSH). Sequence analysis of 1536 ESTs allowed the identification of cDNA clones with similarities with genes involved in plant defense, gene expression regulation and signals transducition pathways. Among the putative genes related with defense machanisms, there is a predominance of genes reported to be regulated by ethylene and jasmonic acid and a lower frequency of ESTs with similarity to genes known to be regulated by salicylic acid. Real time PCR analysis of selected genes revealed that 40% of them are induced in response to Alternaria solani. Transcripts of the majority of these genes were detected between 6 and 12 hai, reaching a maximum value between 24 and 36 hai. Several genes showed a high level of expression and a faster expression response in younger leaves where the disease is less severe. The higher level of defense gene expression associated whith a faster defense response can be one of the mechanisms responsible for the reduced level of early blight observed in yourger tomato leaves.
653 $aAlternaria solani
653 $aEarly blight
773 $tIn: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 13., 2007, Sorrento, Itália. [Proceedings...]. Itália: [s.n.], 2007. p. 321-322.
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